Coding

Part:BBa_K2996001:Design

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-02)
Revision as of 00:08, 11 October 2019 by Pedestal (Talk | contribs) (→‎References)

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cas1-cas2 complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 837
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cas1 and cas2 indeed are seperated by one base pair, as it is in E.coli genome.

Source

The pRec plasmid containing cas1-cas2 is donated from Harris H. Wang from Columbia University. The cas1-cas2 cassette is amplified from NEB 10-beta. They are the only two Cas proteins found in all CRISPR–Cas systems, so cas1-cas2 are pretty conserved.

References

1.Sheth R U , Yim S S , Wu F L , et al. Multiplex recording of cellular events over time on CRISPR biological tape[J]. Science, 2017:eaao0958.

2.Arslan Z , Hermanns V , Wurm R , et al. Detection and characterization of spacer integration intermediates in type I-E CRISPR-Cas system[J]. Nucleic Acids Research, 2014, 42(12):7884-7893.