Coding

Part:BBa_K2952014:Design

Designed by: Maria Fernandez   Group: iGEM19_Edinburgh_UG   (2019-10-10)
Revision as of 19:15, 10 October 2019 by Registry (Talk | contribs)

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Formate dehydrogenase FDHh from Clostridium carboxidivorans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 58
    Illegal NgoMIV site found at 451
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for more efficient expression in E. coli. His-tagged for later purification using immobilised metal affinity chromatography column.


Source

Genomic sequence from Clostridium carboxidivorans, which was codon-optimised for expression in E. coli

References