Composite

Part:BBa_K3037003

Designed by: Arnau Pérez Roig   Group: iGEM19_TU_Dresden   (2019-10-10)
Revision as of 18:59, 10 October 2019 by ArnauP (Talk | contribs)


Fusion protein dCas9 + HRP (MBP/dCas9/linker/HRP/Strep-tag)

The TU Dresden 2019 team design this biobrick in order to create a system for the short and quick detection of specific DNA sequences.

The plasmid used for the expression of the construct was pSB1C3.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2302
    Illegal NheI site found at 5500
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
    Illegal BglII site found at 5742
    Illegal BamHI site found at 4581
    Illegal BamHI site found at 5314
    Illegal XhoI site found at 5826
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


[edit]
Categories
Parameters
None