Part:BBa_K2934001

Invertase-Histag A. niger optimized for B. subtilis

Codes for Invertase enzyme, originally from A. niger, optimized for B. subtilis. The Invertase enzyme catalyzes the degradation of sucrose to glucose and fructose.

Usage and Biology

figure 1: the enzymatic reaction of Invertase

Invertase is an enzyme that catalyzes the cleavage of sucrose into fructose and glucose. Since cleaving the sucrose is the very first step in the creation of honey, we have chosen to use a variation of invertase that is reported to be highly active, which originates from the mold species Aspergillus niger [1]. This type of invertase has been widely investigated and is known to be relatively stable [2]. This invertase works as a dimer [3], each monomer the size of 75 kDa (4) and its optimal pH and temperature are 5 and 60⁰C respectively [3].

References

[1] Nadeem H, Rashid MH, Siddique MH, Azeem F, Muzammil S, Javed MR, Ali MA, Rasul I, Riaz M. 2015. Microbial invertases: A review on kinetics, thermodynamics, physiochemical properties. Process Biochem 50:1202–1210.

[2] Persano Oddo L, Piazza MG, Pulcini P. 1999. Invertase activity in honey. Apidologie 30:57–65.

[3] L’Hocine L, Wang Z, Jiang B, Xu S. 2000. Purification and partial characterization of fructosyltransferase and invertase from Aspergillus niger AS0023. J Biotechnol 81:73–84.

[4] Goosen C, Yuan XL, Van Munster JM, Ram AFJ, Van Der Maarel MJEC, Dijkhuizen L. 2007. Molecular and biochemical characterization of a novel intracellular invertase from Aspergillus niger with transfructosylating activity. Eukaryot Cell 6:674–681.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 863
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1168
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 469
  Illegal BsaI.rc site found at 811
  Illegal SapI.rc site found at 1071