Protein_Domain

Part:BBa_K2934005:Design

Designed by: Nir Litver   Group: iGEM19_Technion-Israel   (2019-10-10)
Revision as of 14:29, 10 October 2019 by NirLitver (Talk | contribs) (→‎References)


Signal peptide-protein linker for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence starts with an EagI (Eco52I) restriction site, for simple substitution od signal peptide. It contains SacI, Eco53kI and NdeI restriction sites.


Source

The part is taken from the pBE-S commercial plasmid made by Takara (https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system)

References

[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system