Device

Part:BBa_K2936018

Designed by: Weng Jiaqi   Group: iGEM19_ZJUT-China   (2019-10-10)
Revision as of 11:13, 10 October 2019 by Registry (Talk | contribs)

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pFrmR-CAT

pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. CAT gene express catalase in the pathway, then the culture added with TMB will turns blue.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1664
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1664
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 907
    Illegal XhoI site found at 1700
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1664
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1664
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None