Coding

Part:BBa_K2967032

Designed by: Shuang Wu   Group: iGEM19_NEU_CHINA   (2019-10-10)
Revision as of 10:24, 10 October 2019 by Hatsuse (Talk | contribs)

YebF-mouse IL10-flag

In our project, we used our engineered bacteria strain to express anti-inflammatory proteins to relieve IBD symptoms. As a complex chronic disorder, IBD can be induced by various factors which we have mentioned before. Among these factors, immunity imbalance is acting as a critical inducer and over-activated immune cells produce large amount of cytokines that lead to intestinal layer destruction and inflammation. However, cytokines production is extremely regulated in immunocompetent host; thus, Interleukin-10 (IL-10) plays a central role in downregulating inflammatory cascades and maintain the intestinal layer functioning [1]. Furthermore, IL-10 has been determined in preventing cell accumulation which consequently alleviates IBD. However, the administration of purified IL-10 protein via oral and intravenous routes is not practical. Therefore, we used genetically modified E. coli strain to express IL-10 protein to relieve chronic inflammation [2].

800px-T--NEU_China--part--il10_mechanism.png

Figure1. The effect of our anti-inflammatory E. coli. Excessive inflammation happens when immune cells such as effector T cells and macrophages keep accumulating. But Interleukin-10 can stop this trend [3].

[1] Steidler, L., et al., Treatment of murine colitis by Lactococcus lactis secreting interleukin-10. Science, 2000. 289(5483): p. 1352-5.

[2] Steidler, L., et al., Biological containment of genetically modified Lactococcus lactis for intestinal delivery of human interleukin 10. Nat Biotechnol, 2003. 21(7): p. 785-9.

[3] Liang YE and Huang Z. IL-10 Signalling in Macrophage during Autoimmunity. Austin Biol. 2016; 1(1): 1004.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 681
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 681
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 681
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 681
  • 1000
    COMPATIBLE WITH RFC[1000]


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