Part:BBa_K2967032
YebF-mouse IL10-flag
In our project, we used our engineered bacteria strain to express anti-inflammatory proteins to relieve IBD symptoms. As a complex chronic disorder, IBD can be induced by various factors which we have mentioned before. Among these factors, immunity imbalance is acting as a critical inducer and over-activated immune cells produce large amount of cytokines that lead to intestinal layer destruction and inflammation. However, cytokines production is extremely regulated in immunocompetent host; thus, Interleukin-10 (IL-10) plays a central role in downregulating inflammatory cascades and maintain the intestinal layer functioning [1]. Furthermore, IL-10 has been determined in preventing cell accumulation which consequently alleviates IBD. However, the administration of purified IL-10 protein via oral and intravenous routes is not practical. Therefore, we used genetically modified E. coli strain to express IL-10 protein to relieve chronic inflammation [2].
Figure1. The effect of our anti-inflammatory E. coli. Excessive inflammation happens when immune cells such as effector T cells and macrophages keep accumulating. But Interleukin-10 can stop this trend [3].
[1] Steidler, L., et al., Treatment of murine colitis by Lactococcus lactis secreting interleukin-10. Science, 2000. 289(5483): p. 1352-5. [2] Steidler, L., et al., Biological containment of genetically modified Lactococcus lactis for intestinal delivery of human interleukin 10. Nat Biotechnol, 2003. 21(7): p. 785-9. [3] Liang YE and Huang Z. IL-10 Signalling in Macrophage during Autoimmunity. Austin Biol. 2016; 1(1): 1004.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 681
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 681
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 681
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 681
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 681
- 1000COMPATIBLE WITH RFC[1000]
| None |