Coding
OriphiC31

Part:BBa_K3254018

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-10)
Revision as of 10:14, 10 October 2019 by Zongyeqing (Talk | contribs)


original sequence of phiC31 integrase gene

This is a conservative design of phiC31 integrase coding sequence. This sequence was isolated from a streptomyces shuttle vector and the EcoRI site was removed. Comparing to BBa_K1039012, this design has a longer coding region, and the "atg" start codon was replaced by "gtg" codon. Because there is an start codon in the upstream, some researchers likely annotated it as a part of the coding sequence. Here we design this part and placed it after a RiboJ(BBa_K1679038) sequence for achieving a appropriate response interval of phiC31 integrase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 75
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1689
    Illegal SapI.rc site found at 1380
    Illegal SapI.rc site found at 1438
    Illegal SapI.rc site found at 1543


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
//function/recombination
Parameters
None