Composite

Part:BBa_K3036007

Designed by: Zhe Feng   Group: iGEM19_BNU-China   (2019-10-10)
Revision as of 09:30, 10 October 2019 by Registry (Talk | contribs)

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Acetic acid overproducing pathway

The natural function of PTA is to reversibly convert acetyl-CoA into acetyl phosphate, whereas that of ACK is to reversibly convert acetate into acetyl phosphate. The trait of PTA, together with the reversibility of the conversion catalyzed by ACK, gives them a potential to enhance acetate production when overexpressed. Moreover, this enhancement can be further optimized when the level of its precursor, acetyl-CoA is lifted. Following this strategy, we include fatty acyl-CoA synthetase, a key enzyme in beta-oxidation of higher fatty acids, to increase the yield of acetate to a further extend.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 615
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1848


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Categories
Parameters
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