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Composite
Part:BBa_K3036007
Designed by: Zhe Feng Group: iGEM19_BNU-China (2019-10-10)
Acetic acid overproducing pathway
The natural function of PTA is to reversibly convert acetyl-CoA into acetyl phosphate, whereas that of ACK is to reversibly convert acetate into acetyl phosphate. The trait of PTA, together with the reversibility of the conversion catalyzed by ACK, gives them a potential to enhance acetate production when overexpressed. Moreover, this enhancement can be further optimized when the level of its precursor, acetyl-CoA is lifted. Following this strategy, we include fatty acyl-CoA synthetase, a key enzyme in beta-oxidation of higher fatty acids, to increase the yield of acetate to a further extend.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 615
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1848
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Categories
Parameters
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