Device
Cry11Aa Pl

Part:BBa_K2938015

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-10-09)
Revision as of 09:16, 10 October 2019 by Assafi (Talk | contribs)


Cry11Aa (Strep tag) + deGFP Device

PR1 - RBS - Cry11Aa(Strep tag) - RBS - deGFP - T500 Terminator

This plasmid was planned on a pBEST plasmid backbone. It contains larvicidal activity, as it containing toxic subunit of the bacillus thuringiensis israelensis (BTI) pBtoxis plasmid which is toxic to mosquitoes larva.

The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1870
    Illegal XbaI site found at 2116
    Illegal SpeI site found at 982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1870
    Illegal NheI site found at 56
    Illegal NheI site found at 105
    Illegal SpeI site found at 982
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1870
    Illegal XhoI site found at 2849
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1870
    Illegal XbaI site found at 2116
    Illegal SpeI site found at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1870
    Illegal XbaI site found at 2116
    Illegal SpeI site found at 982
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None