Part:BBa_K2978000
Causes cell lysis of the pathogen Clostridium difficile
This part makes references to an endolysin from a bacteriophage that infects Clostridium difficile. It is section of the complete protein CD27L, which in the residues 2 to 143 has its domain homologous to N-acetyl-muramoyl-L-alanine amidases. In this part we synthesize de novo the residues 1 to 179 of the protein, with codon optimization for E.coli. In previously works this reduced protein has shows a faster lysis to different strains of the pathogen and a high level of selectivity against clostridia (Wang, et al.; 2015).
Usage and Biology
The expression of this parts is under a T7 promoter, this mean that if we want to induce this protein we have to use a chassis with T7. In our case we confirm that E. coli BL21 de3 produce the lysin when IPTG is applied, because in this strain the polymerase is under a pLac promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| device_type | |
| positive_regulators | |
| protein |