Composite

Part:BBa_K2967030:Design

Designed by: Rui Wang   Group: iGEM19_NEU_CHINA   (2019-10-09)
Revision as of 02:22, 10 October 2019 by Wangrui (Talk | contribs) (→‎References)

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NorR-PnorV-amplicp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 867
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 642


Design Notes

This part can be used to detect the nitric oxide in the environment.

Source

The promoter PnorV comes from the genome of K12 strain. The NorR、RBS and blue chromoprotein come from registry.

References

[1] Rachmilewitz D, Stamler J S, Bachwich D, et al. Enhanced colonic nitric oxide generation and nitric oxide synthase activity in ulcerative colitis and Crohn's disease[J]. Gut, 1995, 36(5): 718-723.

[2] Ljung T, Herulf M, Beijer E, et al. Rectal nitric oxide assessment in children with Crohn disease and ulcerative colitis. Indicator of ileocaecal and colorectal affection[J]. Scandinavian journal of gastroenterology, 2001, 36(10): 1073-1076.

[3] Tucker, N. P., D’Autreaux, B., Yousafzai, F. K., Fairhurst, S. A., Spiro, S., and Dixon, R. (2008) Analysis of the nitric oxide-sensing non-heme iron center in the NorR regulatory protein. J. Biol. Chem. 283, 908−918.

[4] Bush, M., Ghosh, T., Tucker, N., Zhang, X., and Dixon, R. (2011) Transcriptional regulation by the dedicated nitric oxide sensor, NorR: a route towards NO detoxification. Biochem. Soc. Trans. 39, 289−293.

[5] Archer, E.J., Robinson, A.B. & Suel, G.M. Engineered E. coli that detect and respond to gut inflammation through nitric oxide sensing. ACS Synth. Biol. 1, 451–457 (2012).