Device
Part:BBa_K2938016
Designed by: Assaf Vital Group: iGEM19_BGU_Israel (2019-10-09)
Cry11Aa (Strep tag) + P20 (His tag) + deGFP Device
PR1 - RBS - Cry11Aa (Strep tag) - RBS - P20 (His tag) - RBS - deGFP (His tag) - T500 Terminator
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1870
Illegal EcoRI site found at 2426
Illegal XbaI site found at 2116
Illegal XbaI site found at 2761
Illegal SpeI site found at 982 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1870
Illegal EcoRI site found at 2426
Illegal NheI site found at 56
Illegal NheI site found at 105
Illegal SpeI site found at 982 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1870
Illegal EcoRI site found at 2426
Illegal XhoI site found at 3494 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1870
Illegal EcoRI site found at 2426
Illegal XbaI site found at 2116
Illegal XbaI site found at 2761
Illegal SpeI site found at 982 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1870
Illegal EcoRI site found at 2426
Illegal XbaI site found at 2116
Illegal XbaI site found at 2761
Illegal SpeI site found at 982 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |