Composite
IBR-C35
Part:BBa_K3254000:Design
Designed by: Yeqing Zong Group: iGEM19_GENAS_China (2019-10-09)
Revision as of 08:51, 9 October 2019 by Zongyeqing (Talk | contribs)
phiC31attB-BsaI sites-terminator-phiC31attP(r)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 59
Illegal BsaI.rc site found at 47
Design Notes
This part sequence was tested in silico before synthesised to avoid potential promoter activity (see the website in references). It also should be noted that the sequence of attP site (BBa_K2460011) was reversed.
Source
This part was totally synthesised by commerical company.
References
Chen, Y.J., et al., Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods, 2013. 10(7): p. 659-64. http://www.fruitfly.org/seq_tools/promoter.html