Coding

Part:BBa_K2936001:Design

Designed by: Weng Jiaqi   Group: iGEM19_ZJUT-China   (2019-10-08)
Revision as of 13:52, 8 October 2019 by Registry (Talk | contribs)

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pBAD-LacZɑ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
    Illegal BamHI site found at 182
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The β-Gal is very sensitive with ONPG. Besides, ONPG has a benzene ring, attention should be paid when testing with uv spectrophotometer.


Source

The pGLO plasmid

References