Coding
Part:BBa_K2934001:Design
Designed by: Nir Litver, Lior Haim Group: iGEM19_Technion-Israel (2019-10-08)
Invertase-Histag A. niger optimized for B. subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 863
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1168
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 469
Illegal BsaI.rc site found at 811
Illegal SapI.rc site found at 1071
Design Notes
The sequence is based on the A. niger Invertase amino acid sequence and codon optimization for B. subtilis, using codon usage tables [2] [3].
Source
The sequence is based on the Invertase amino acid sequence [1] and is optimized for B. subtilis.
References
[1] L. M. Boddy, T. Berges, C. Barreau, M. H. Vainstein, M. J. Dobson, D. J. Ballance, j. E Peberdy. 1993. Purification and characterisation of an Aspergillus niger invertase and its DNA sequence, Current Genetics.
[2] https://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1423
[3] http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=224308&aa=1&style=N