Coding

Part:BBa_K2934000:Design

Designed by: Nir Litver, Lior Haim   Group: iGEM19_Technion-Israel   (2019-10-07)
Revision as of 12:39, 8 October 2019 by NirLitver (Talk | contribs) (→‎References)


Glucose Oxidase-Histag A. niger optimized for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 30
    Illegal BsaI site found at 361
    Illegal BsaI.rc site found at 307
    Illegal BsaI.rc site found at 1132


Design Notes

The sequence is based on the A. niger glucose oxidase (GOx) amino acid sequence and codon optimization for B. subtilis, using codon usage tables [2] [3].

Source

The amino acid sequence was taken from UniProt [1], the gene sequence is based on the amino acid sequence.

References

[1] https://www.uniprot.org/uniprot/P13006

[2] https://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1423

[3] http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=224308&aa=1&style=N