Coding

Part:BBa_K2934001:Design

Designed by: Nir Litver, Lior Haim   Group: iGEM19_Technion-Israel   (2019-10-08)
Revision as of 10:11, 8 October 2019 by NirLitver (Talk | contribs) (→‎References)


Invertase-Histag A. niger optimized for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 863
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 469
    Illegal BsaI.rc site found at 811
    Illegal SapI.rc site found at 1071


Design Notes

The sequence is based on the A. niger Invertase amino acid sequence and codon optimization for B. subtilis, using codon usage tables (https://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1423, http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=224308&aa=1&style=N).


Source

The sequence is based on the Invertase amino acid sequence [1] and is optimized for B. subtilis.

References

[1] L. M. Boddy, T. Berges, C. Barreau, M. H. Vainstein, M. J. Dobson, D. J. Ballance, j. E Peberdy. 1993. Purification and characterisation of an Aspergillus niger invertase and its DNA sequence, Current Genetics.