Part:BBa_K2967013
Amplifier system with hIL-10.
Amplifier system is created by our team NEU-CHINA 2019. To make sure that amplifier is able to strengthen the expression of gene downstream to promoter hrpL. A coomassie brilliant blue is demonstrated to show that more protein at 35kDA, which should be IL-10, is witnessed when introducing IL-10 gene downstream of promoter hrpL. And it can be seen that lanes with amplifier seems to be darker, which means that this amplifier system tends to strengthen overall protein expression.
Figure 1. Diagram of expressing vector. HrpR and HrpS amplifier system is used as a booster to boost the expression level of gene downstream promoter hrpL. Protein HrpR and HrpS can bind together to induce the PhrpL which strengthen the expression of downstream gene YebF-IL10.
Figure 2. Expressing vector is transformed into BL21 strain. CFU is then inoculated to LB medium followed by 12h incubation at 37℃. After induction of IPTG, final concentration at 1mM, the culture is incubated at 37℃ for 2h. A coomassie brilliant blue is demonstrated to show the result. The red box shows where the target protein is.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2082
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1292
Illegal SapI.rc site found at 1925
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