Composite
Part:BBa_K3102023
Designed by: Sarah DUQUESNE Group: iGEM19_Ionis_Paris (2019-10-07)
Revision as of 08:31, 8 October 2019 by ChloeDOIZELET (Talk | contribs)
T7-CAE-ter (secreted CAE)
This T7-CAE-TEV-hlyA-ter (secreted CAE) composite is engaged into the cellulose acetate esterase (CAE) synthetization with the CAE part (BBa_K3102010) and secretion thanks to TEV (BBa_K3102012) and HlyA basic parts (BBa_K554002). The CAE is allowed to break down the cellulose acetate fibers. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV part added a TEV recognition sequence, that will be used to remove the signal peptide, HlyA.
This constructs is enriched with primer, T7 promoters, RBS and terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 124
Illegal NgoMIV site found at 1234
Illegal AgeI site found at 254
Illegal AgeI site found at 341
Illegal AgeI site found at 479
Illegal AgeI site found at 662
Illegal AgeI site found at 710
Illegal AgeI site found at 878
Illegal AgeI site found at 1091 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |