Part:BBa_K3071000
Sensory/regulatory protein RpfC from Xanthomononas campestris pv. campestris
RpfC is a kinase located in the cell membrane of bacteria acting as a sensor. It consists of three domains, including the input domain, histidine kinase (HisKA)domain, CheY-like two-component receiver domain (REC) and the C-terminal histidine phosphotransfer (HPT) domain. The sensory domain is composed of 5 transmembrane helices with periplasmic and cytoplasmic loops less than 20 amino acids long. When the DSF signal is received, active autophosphorylation starts at the His198 of HisKA domain. It will then be followed by a phosphorelay through the Asp512 and His657 in the REC domain and the HPT domain respectively. The phosphotransfer lastly activates the RpfG by phosphorylation at the REC domain of RpfG.(figure 1) Besides activating the RpfG signal protein, the second role of RpfC is to interact with RpfF, a DSF synthase, by its REC domain, in order to suppress the DSF production in that bacterial cell.
Research showed that point mutations at His 198, Asp512 or His657 resulted in the reduction of virulence factors, extracellular cellulases, and proteases, demonstrating the importance of these three amino acids in the process of RpfC-RpfG signaling and the role of RpfC in virulence factor production.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 303
Illegal AgeI site found at 1135
Illegal AgeI site found at 1614
Illegal AgeI site found at 1698 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 352
Illegal BsaI.rc site found at 216
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