Composite

Part:BBa_K2967026:Design

Designed by: Rui Wang   Group: iGEM19_NEU_CHINA   (2019-10-03)
Revision as of 15:40, 7 October 2019 by Wangrui (Talk | contribs) (→‎References)


The yeaR promoter added an extra NsrRBSs-RBS-Luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 228
    Illegal NgoMIV site found at 1572
    Illegal NgoMIV site found at 1593
    Illegal AgeI site found at 1296
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1478


Design Notes

To reduce sensor basal background, we inserted an extra NsrR binding sequence (NsrRBS) downstream of PyeaR to create a ‘roadblocking’ effect. The distance between the two NsrRs , the sensor’s leakiness and sensitivitcan be adjusted. However, due to time limitation we only modified 47 bp between two NsrRBSs.

Source

None

References

Merulla, D. & van der Meer, J. R. Regulatable and modulable background expression control in prokaryotic synthetic circuits by auxiliary repressor binding sites. ACS Synth. Biol. 5, 36–45 (2016).