Coding

Part:BBa_K3185004

Designed by: Masahiro Sakono   Group: iGEM19_Kyoto   (2019-10-04)
Revision as of 13:52, 7 October 2019 by 0012massan (Talk | contribs)


SPYCatcher -> BaCBM2

BaCBM2 is a Carbonhydrate-binding Module family 2(CBM2) derived from Bacillus anthracis[1].

CBMs are protein domains mainly found in carbohydrate-active enzymes.

In this part, we express BaCBM2 gene downstream of SPY Catcher gene(りんく).

Using this part with any protein linked with SPY tag, you can anchor this part to your objective protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None