Composite

Part:BBa_K3205000

Designed by: Shengkun Tong   Group: iGEM19_HZAU-China   (2019-10-07)
Revision as of 13:43, 7 October 2019 by Registry (Talk | contribs)

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A device for detection benzyl alcohol by producing GFP.

AreR is a member of the NtrC/XylR regulatory protein family, and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ^54-type enhancer protein can binding to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream gene transcription. In this part, we express AreR with J23102 constituent promoter, regulated GFP expression with Pare. A significant difference in fluorescence intensity were observed under different concentrations of benzyl alcohol. This part can be used for the detection of benzyl alcohol or other designs requiring benzyl alcohol induction. You can also replace GFP with other genes for other signaling pathways that require benzyl alcohol induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 66
    Illegal BglII site found at 712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2772


[edit]
Categories
//awards/composite_part
//function/reporter/fluorescence
//function/sensor
//regulation/multiple
Parameters
None