Composite
Part:BBa_K2959007:Design
Designed by: Alejandro Aguirre Hernndez Group: iGEM19_Tec-Chihuahua (2019-10-07)
Expressible Wheat antimicrobial peptide 1b
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 261
Design Notes
The sequence of WAMP1b is preceded by a 6x His-Tag to facilitate its purification by immobilized metal affinity chromatography.
Source
The composite part was designed using the following parts previously added to the Registry: BBa_R0010, BBa_B0034, and BBa_B0015. The amino acid sequence for WAMP1b was retrieved from Odintsova et al., 2009.