Scar

Part:BBa_K2996602

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-05)
Revision as of 04:04, 7 October 2019 by Pedestal (Talk | contribs)


scar between RSRL and EGFP

After constructing pGEX4t-EGFP, we gave it to company to synthesize our desired RSRL sequence. Since the fragment can only be put between restrcition sites, there is a scar left between RSRL and EGFP. Actually, it is the restrcition site for BamHⅠ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

<img style="max-width: 50%" src="T--TUDelft--2018_Figure1_colonyPCR_linkerTn5_Fusion.png" alt="" />


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Parameters
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