Other

Part:BBa_K2996700

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-06)
Revision as of 03:57, 7 October 2019 by Pedestal (Talk | contribs)


Flexible Linker 4 that separates RpoA and dCas9

Flexible Linker 4 (FL4) has been proved to be effectively separate bifunction proteins. This is a long linker (25 aa), which better accommodates RpoA:promoter interaction. Amino acid sequence of FL4 is:LSGGGGSGGGGSGGGGSGGGGSGGGGSAAA. It links RpoA and dCas9.

Usage and Biology

12345


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None