Part:BBa_K2957001
IL-8 (CXCL-8) with FLAG Tag
Contents: IL-8 (CXCL-8), Flag Tag, with Carb resistance
What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)
What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It has a Flag tag attached.
How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allow for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page on our MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts. It also has a Flag tag attached to it (which makes it different from pIG_048, BBa K779200) so this allows it to be tracked and then blotted with ELISA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 244
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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