Coding

Part:BBa_K3098017

Designed by: Junfan Chen   Group: iGEM19_WHU-China   (2019-10-06)
Revision as of 16:07, 6 October 2019 by Registry (Talk | contribs)

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Strep-dCBM3a

This part is composed by the dCBM3a which is the improved dimer of the CBM3a (corrected version of the Part:BBa_K1321014) fused at the C-terminus and the monomeric streptavidin ( corrected version of the part:BBa_K1896000) at the N-terminus. The dCBM3a domain has a high affinity for the bacterial cellulose. And the monomeric strepvidin domain can bind biotinylated compounds. The part can act as a adaptor which makes the binding of biotinylated functional compounds and the bacterial cellulose possible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1008
    Illegal NgoMIV site found at 1026
    Illegal NgoMIV site found at 1044
    Illegal NgoMIV site found at 1596
    Illegal NgoMIV site found at 1614
    Illegal NgoMIV site found at 1632
    Illegal AgeI site found at 19
    Illegal AgeI site found at 85
    Illegal AgeI site found at 361
    Illegal AgeI site found at 790
    Illegal AgeI site found at 993
    Illegal AgeI site found at 1378
    Illegal AgeI site found at 1581
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None