Coding

Part:BBa_K3044008:Design

Designed by: Catharina Bang Jensen   Group: iGEM19_SDU-Denmark   (2019-10-06)
Revision as of 12:08, 6 October 2019 by Registry (Talk | contribs)

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dCas9 codon optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the optimization illegal restriction sites were avoided. Aother thing to keep in mind was to figure out wich codons to choose for E. coli K12


Source

The original sequence is from BBa_K1150000 and was codon optimized based on a codon frequency table [1]

References