Coding
Part:BBa_K3044008:Design
Designed by: Catharina Bang Jensen Group: iGEM19_SDU-Denmark (2019-10-06)
dCas9 codon optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 1325 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
During the optimization illegal restriction sites were avoided. Aother thing to keep in mind was to figure out wich codons to choose for E. coli K12
Source
The original sequence is from BBa_K1150000 and was codon optimized based on a codon frequency table [1]