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Part:BBa_K2996700:Design

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-06)
Revision as of 07:02, 6 October 2019 by Registry (Talk | contribs)

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Flexible Linker 4 that separates RpoA and dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Highly-repeating sequence is difficult to deal with during molecular cloning and overlap extension PCR during two trials both end up in failure. So, we optimized FL4 sequence ourselves, with a simple Python program that randomly select codons for FL4.



Source

None.

References