Coding

Part:BBa_K2996703:Design

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-09-16)
Revision as of 06:44, 6 October 2019 by Pedestal (Talk | contribs)


RpoA, DNA-directed RNA polymerase subunit alpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 459
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

C-terminal is fused to dcas9 through a flexible linker.


Source

This sequence is amplified from E. coli K12 genome.

References

1.Bikard D , Jiang W , Samai P , et al. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system[J]. Nucleic Acids Research, 2013, 41(15):7429-7437.