DNA

Part:BBa_K3090002

Designed by: Jungwoo Choe   Group: iGEM19_Korea_HS   (2019-10-06)
Revision as of 06:32, 6 October 2019 by Registry (Talk | contribs)

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single chain variable fragment (scFv(P5)) with cpp

scFv(P5) is chimeric molecule in which several groups of residues important for antigen binding in the poorly stable anti-hen egg lysozyme (HEL) scFv(D1.3) were progressively grafted onto the scFv(F8) scaffoldis to maintain cytoplasmic stability and specificity. Cell penetrating peptide from Porcine circovirus type 2 was fused to the N-terminus of scFv(P5) for cell penetration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 306


[edit]
Categories
//collections/immune_regulation/antibodies
Parameters
None