Coding
Part:BBa_K2996703:Design
Designed by: Yunqing Wang Group: iGEM19_SJTU-BioX-Shanghai (2019-09-16)
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Design Notes
C-terminal is fused to dcas9 through a flexible linker.
Source
This sequence is amplified from E. coli.
References
1.Bikard D , Jiang W , Samai P , et al. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system[J]. Nucleic Acids Research, 2013, 41(15):7429-7437.