Coding
Part:BBa_K3139013:Design
Designed by: Zhen Cao Group: iGEM19_NAU-CHINA (2019-10-05)
Optimized anti-plasmodium fusion protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 409
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
With TEV protease recognition and cleavage sites added between each 2 peptide coding domains, the fusion protein could be cleaved with TEV protease and each peptide effects separately. And with this design, the function of our fusion protein could be more easily stimulated than using a complete fusion one (with no or part cleavage), since we got the effects of each single peptide but not the fusion one.
Source
The source of this part has been described in each page of the basic parts consisted.