Coding

Part:BBa_K3139013

Designed by: Zhen Cao   Group: iGEM19_NAU-CHINA   (2019-10-05)
Revision as of 16:02, 5 October 2019 by Registry (Talk | contribs)

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Optimized anti-plasmodium fusion protein

The optimized anti-plasmodium fusion protein contains all the anti-plasmodium peptides uploaded (BBa_K3139000, BBa_K3139002, BBa_K3139003, BBa_K3139004, BBa_K3139005, BBa_K3139006, BBa_K3139007, BBa_K3139008, BBa_K3139009), TEV protease recognition and cleavage sites (BBa_K3139011) between each 2 peptide coding domains, and a His-tag is attached on the N-terminal for purification. They were found in some reviews in this field and chosen by our team members as their efficient effect reported. With reports of each sigle peptide, we primarily acquire the anti-plasmodium effect of each peptide. And we finally confirmed its sequence with help from mathmatical model. The copies and types of the peptides were selected elaborately.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 409
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None