Composite
Part:BBa_K3111201
Designed by: Matas Deveikis Group: iGEM19_UCL (2019-09-18)
mScarlet_DARPin929_StrepII
This part encodes mScarlet_DARPin929. The use of this part enabled us to study the binding and uptake of DARPin929 by conducting cell culture studies on SK-BR-3 HER2+ cells. The DARPin is flanked by red fluorescent protein mScarlet on the N-terminus which allowed visualisation of binding under confocal microscopy and a StrepII-tag downstream of this fusion, which allows for column-based purification of the expressed protein. It is expressed under a T7 promoter and a strong RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 791
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 652
- 1000COMPATIBLE WITH RFC[1000]
Experimental Results
[edit]
Categories
Parameters
None |