Coding
TALEsp1

Part:BBa_K2918008

Designed by: TUDelft2019   Group: iGEM19_TUDelft   (2019-10-05)
Revision as of 13:41, 5 October 2019 by Registry (Talk | contribs)

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Transcription Activator like Effector protein (TALEsp1)

Usage and Biology: Transcription Activator Like Effectors (TALE) proteins were originally discovered in a plant pathogenic bacterial genus Xanthomonas (Doyle, Stoddard et al. 2013). These proteins consists of repeats where 12th and 13th amino acids can vary, these are called the repeat variable diresidue (RVD). These RVDs have been shown to bind to DNA in a simple one-to-one binding code (Doyle, Stoddard et al. 2013):

Amino acids Target HD C NG T NN G NI A

The direct correspondence between amino acids allows scientists to engineer these repeat regions to target any sequence they want.

In our system we used the TALE protein as a repressor by engineering promoters to contain the binding site of this specific TALE protein.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 235
    Illegal PstI site found at 2490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 235
    Illegal PstI site found at 2490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 202
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 235
    Illegal PstI site found at 2490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 235
    Illegal PstI site found at 2490
    Illegal AgeI site found at 1229
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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