Composite

Part:BBa_K2967013

Designed by: Shuang Wu   Group: iGEM19_NEU_CHINA   (2019-09-30)
Revision as of 06:08, 5 October 2019 by Wangrui (Talk | contribs)


Amplifier system with hIL-10.

Amplifier system is created by our team NEU-CHINA 2019. To make sure that amplifier is able to strengthen the expression of gene downstream to promoter hrpL. A coomassie brilliant blue is demonstrated to show that more protein at 35kDA, which should be IL-10, is witnessed when introducing IL-10 gene downstream of promoter hrpL. And it can be seen that lanes with amplifier seems to be darker, which means that this amplifier system tends to strengthen overall protein expression.

800px-T--NEU_China--part--amp%2Bil-10-1.png

Figure 1. Expressing vector is transformed into BL21 strain. CFU is then inoculated to LB medium followed by 12h incubation at 37℃. After induction of IPTG, final concentration at 1mM, the culture is incubated at 37℃ for 2h. A coomassie brilliant blue is demonstrated to show the result. The red box shows where the target protein is.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1292
    Illegal SapI.rc site found at 1925


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