Coding
katG

Part:BBa_K137068:Experience

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-07-31)
Revision as of 00:09, 5 September 2008 by Jkm (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K137068

KatG+LAA is functional

  • Construct: K137079
  • Strain: SN0037 (ΔkatE, katG)
  • Media: M9 + 2% glucose
  • KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.


Hydrogen peroxide (mM) +AHL (100nM) -AHL
0.0 Y Y
0.3 Y Y
1.0 Y N
3.0 Y N
10.0 N N

Y = Turbid growth after overnight incubation. N = No turbid growth after overnight incubation.

User Reviews

UNIQb63c283899d8bf40-partinfo-00000000-QINU UNIQb63c283899d8bf40-partinfo-00000001-QINU