Composite

Part:BBa_K3098005

Designed by: Yiru Shen   Group: iGEM19_WHU-China   (2019-10-04)
Revision as of 18:03, 4 October 2019 by Etzel (Talk | contribs)


Cellulose Synthase Operon with T5 promoter

Cellulose GxCesA and GxCesB, with pT5 promoter, two RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 655
    Illegal BglII site found at 1217
    Illegal BamHI site found at 4346
    Illegal XhoI site found at 307
    Illegal XhoI site found at 3729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
    Illegal NgoMIV site found at 287
    Illegal AgeI site found at 2268
    Illegal AgeI site found at 4401
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4172
    Illegal BsaI.rc site found at 1792


[edit]
Categories
Parameters
None