Composite

Part:BBa_K3221202:Design

Designed by: Hao Zhou   Group: iGEM19_SCU-China   (2019-10-04)
Revision as of 14:54, 4 October 2019 by Kerry (Talk | contribs) (→‎Source)

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pMET3-yeGFP-CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 399
    Illegal BsaI.rc site found at 1144


Design Notes

We designed this part to fit the RFC10.

Source

We got the pMET3 from the BY4742 genomic, and the plasmid with yeGFP from the Team Peking. The CYC1 terminator is initially on the pYES2.

References