Coding
katG
Part:BBa_K137068:Experience
Designed by: Doug Tischer Group: iGEM08_Caltech (2008-07-31)
Revision as of 19:43, 4 September 2008 by Tischerd (Talk | contribs) (→Applications of BBa_K137068)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K137068
- KatG+LAA is functional
- Strain: SN0037 (DE katE, katG)
- Media: M9 + 2% glucose
- KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.
| Hydrogen peroxide (mM) | +AHL (100mM) | -AHL |
|---|---|---|
| 0.0 | Y | Y |
| 0.3 | Y | Y |
| 1.0 | Y | N |
| 3.0 | Y | N |
| 10.0 | N | N |
Y = Turbid growth after overnight incubation. N = No turbid growth after overnight incubation.
User Reviews
UNIQ9b1f2e783965c48c-partinfo-00000000-QINU UNIQ9b1f2e783965c48c-partinfo-00000001-QINU