Coding

Part:BBa_K3102011:Design

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 21:06, 3 October 2019 by Registry (Talk | contribs)

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EG1 (Endo-1,4-beta-glucanase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
    Illegal AgeI site found at 313
    Illegal AgeI site found at 403
    Illegal AgeI site found at 1240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence synthesized and optimized for E.coli on Geneius



Source

Neisseria Sicca, sequence found through genebank https://www.ncbi.nlm.nih.gov/protein/BAJ21449.1

References