Measurement

Part:BBa_K2970010

Designed by: Alexander Bast   Group: iGEM19_Hamburg   (2019-10-03)
Revision as of 18:37, 3 October 2019 by Registry (Talk | contribs)

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J23102 Test Composition with RiboJ

Since the RBS can influence the promoter strength, it may be necessary to separate it from the promoter. For this we used RiboJ, which always makes the same cut in front of the promoter. Thus this plasmid can be used to measure promoters. In this case the promoter J23102 was inserted and measured.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 789


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