Coding

Part:BBa_K3102022

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)
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ACS (Acetyl-coenzyme A synthetase) Shewanella Oneidensis

ACS (Acetyl-coenzyme A synthetase) activity constitutes one of two distinct pathways by which E. coli activates acetate to acetyl-CoA in an ATP-dependent manner. [Brown et al., 1977; Kumari et al., 1995] The ACS pathway functions in a mainly anabolic role, scavenging acetate present in the extracellular medium. The ACS gene encoding this enzyme is overexpressed to enhance acetyl-CoA's generation.

Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36. Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 931
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None