Coding

Part:BBa_K3037001

Designed by: Arnau Pérez Roig   Group: iGEM19_TU_Dresden   (2019-10-03)
Revision as of 15:43, 3 October 2019 by Registry (Talk | contribs)

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Maltose Binding Protein (MBP-tag)

Maltose binding protein is a 42 kDa Protein. It is a well-established, reliable protein-tag that can increase the solubility of proteins it is fused to, therefore limits the risk of accumulation of overexpressed recombinant protein in inclusion bodies and increases the total protein yield. It can also improve expression of difficult enzymes like Cas9. It can further be used to purify proteins via affinity chromatography in amylose resin.(1) To make it easy to translationally fuse this BioBrick to any protein of interest it is provided in RCF25. (2) Additionally we included a pre-scission site upstream and downstream of the coding sequence, so the tag can be easily removed by purification in a digest. Additionally the pre-siccon sequence acts as a linker and thereby limits the risk of steric hindrance.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


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Categories
Parameters
None