Regulatory

Part:BBa_K3098022

Designed by: Liangyue Song   Group: iGEM19_WHU-China   (2019-10-03)
Revision as of 12:46, 3 October 2019 by Registry (Talk | contribs)

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Promoter(regulated by cI and lacI)

This promoter, improved from the part BBa_R0051, has become lacI repressible and thus IPTG inducible with the doubled lacO gene (lacI binding site) after the previous promoter. This new promoter has two binding sites for cI and two binding site for lacI. Hence, this new promoter can be regulated by two signals simultaneously: only when IPTG+/cI-, the coding sequence behind this promoter can be transcribed , leading to more potential uses in the design of gene circuits.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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