Composite

Part:BBa_K2967023

Designed by: Lijun Bao   Group: iGEM19_NEU_CHINA   (2019-10-02)
Revision as of 04:56, 3 October 2019 by Registry (Talk | contribs)

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PytfE-RBS-Luc

Nitrate and Nitrite sensitive promoter PytfE with a reporter gene luciferasen and strong RBS to create a nitrate-sensitive system which signals through expression of Luc.

PytfE is normally repressed by NsrR, a protein native to most 'E. coli' cells. When Nitrate or Nitrite enter the cell it is converted to Nitric Oxide. This binds to NsrR, halting the repression and allowing the production of Luc.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 194
    Illegal NgoMIV site found at 1538
    Illegal NgoMIV site found at 1559
    Illegal AgeI site found at 1262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1444


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Parameters
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